Customized DNA-extraction from clay materials
Hands-on training on applying the customized DNA-extraction protocol developed at SCK•CEN on clay samples. Exchange opportunity for a PhD student or Professional. The candidate needs to have a background in Molecular lab techniques.
Introduction
Concerning the extraction and downstream analysis of microbial metagenomes, it has been shown that the extraction efficiency can be quite low in samples rich in clay and/or organic matter. Also, the method of DNA-extraction seems to impact the output (the microbial species of which the DNA was actually extracted), meaning that different extractions can target different microbial species. We therefore strive to use one efficient protocol for all applications (both water and clay samples) to enable proper comparison between samples and have developed an in-house protocol to do so.
Objectives
• Gain insight in the different steps of DNA-extraction
• Perform the DNA-extraction protocol on reference samples
• Perform the DNA-extraction on own samples (optional)
• Gain insight in the different steps of DNA-extraction
Duration
One week, but can be longer if own samples are brought along – to be discussed.
The Candidate
Exchange opportunity for a PhD student or Professional. The candidate needs to have a background in Molecular lab techniques.
Practical modalities
Accomodation (including bicycle) can be foreseen at the SCK•CEN dormitories.
Application modalities, other than those specified below
Continuously application possible. Need to apply three months in advance, to arrange access to the SCK•CEN domain and laboratories.
Additional information
Host: SCK•CEN
Country: Belgium
Language: English
Mentor: Katinka Wouters, Hugo Moors
Contact email: katinka.wouters[at]sckcen.be
Website: http://science.sckcen.be/en/Institutes/EHS/MCB/MIC
MIND reference: MIND01
Host reference: MIND-SCK-1
Applications should be submitted by email to mind.project[at]sckcen.be and should contain
– a motivation letter with MIND-reference to the opportunity as mentioned on this website;
– a CV of the candidate.
Approval of your request will be subject to the evaluation of your application file by a Jury composed by members of the MIND project WP3, as well as the host institution. You will be informed about the status of your application via email.
In case of questions, please contact us via mind.project[at]sckcen.be and always mention the MIND reference number of the opportunity.